Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene of hemicellulose/pectin formation.
Identifieur interne : 004556 ( Main/Exploration ); précédent : 004555; suivant : 004557Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene of hemicellulose/pectin formation.
Auteurs : Henrik Johansson [Suède] ; Fredrik Sterky ; Bahram Amini ; Joakim Lundeberg ; Leszek A. KleczkowskiSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2002.
Descripteurs français
- KwdFr :
- ADN complémentaire (biosynthèse), ADN complémentaire (composition chimique), ADN complémentaire (isolement et purification), Arbres (MeSH), Banque de gènes (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Feuilles de plante (métabolisme), Gènes de plante (MeSH), Paroi cellulaire (métabolisme), Pectine (métabolisme), Polyosides (métabolisme), Polyéthylène glycols (MeSH), Saccharose (MeSH), Sites de fixation (MeSH), Sorbitol (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), UDP glucose dehydrogenase (biosynthèse), UDP glucose dehydrogenase (génétique).
- MESH :
- biosynthèse : ADN complémentaire, UDP glucose dehydrogenase.
- composition chimique : ADN complémentaire.
- génétique : UDP glucose dehydrogenase.
- isolement et purification : ADN complémentaire.
- métabolisme : Feuilles de plante, Paroi cellulaire, Pectine, Polyosides.
- Arbres, Banque de gènes, Clonage moléculaire, Données de séquences moléculaires, Gènes de plante, Polyéthylène glycols, Saccharose, Sites de fixation, Sorbitol, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Binding Sites (MeSH), Cell Wall (metabolism), Cloning, Molecular (MeSH), DNA, Complementary (biosynthesis), DNA, Complementary (chemistry), DNA, Complementary (isolation & purification), Gene Library (MeSH), Genes, Plant (MeSH), Molecular Sequence Data (MeSH), Pectins (metabolism), Plant Leaves (metabolism), Polyethylene Glycols (MeSH), Polysaccharides (metabolism), Sorbitol (MeSH), Sucrose (MeSH), Trees (MeSH), Uridine Diphosphate Glucose Dehydrogenase (biosynthesis), Uridine Diphosphate Glucose Dehydrogenase (genetics).
- MESH :
- chemical , biosynthesis : DNA, Complementary, Uridine Diphosphate Glucose Dehydrogenase.
- chemical , chemistry : DNA, Complementary.
- chemical , genetics : Uridine Diphosphate Glucose Dehydrogenase.
- chemical , isolation & purification : DNA, Complementary.
- metabolism : Cell Wall, Pectins, Plant Leaves, Polysaccharides.
- Amino Acid Sequence, Base Sequence, Binding Sites, Cloning, Molecular, Gene Library, Genes, Plant, Molecular Sequence Data, Polyethylene Glycols, Sorbitol, Sucrose, Trees.
Abstract
Plant UDP-glucose dehydrogenase (UGDH) is an important enzyme in the formation of hemicellulose and pectin, the components of newly formed cell walls. A cDNA clone (Ugdh) corresponding to UGDH was isolated from a cDNA library prepared from cambial zone of poplar (Populus tremula x tremuloides). Within the 1824-nucleotide (nt)-long clone, an open reading frame encoded a protein of 481 amino acids (aa), with a calculated molecular weight of 53.1 kDa. The derived aa sequence showed 90% and 63% identity with UGDHs from soybean and bovine liver, respectively, and had highly conserved aa motifs believed to be of importance for nt binding and catalytic efficiency. In poplar, the Ugdh corresponds to one or two genes, as found by genomic Southern analysis. The gene was expressed predominantly in differentiating xylem and young leaves, with little expression in the phloem zone of the stem. The expression pattern matched that of UGDH protein, as found by immunoblotting. In leaves, the Ugdh expression was upregulated by a short-term feeding with sucrose, sorbitol and polyethylene glycol, and this effect was to some extent mimicked by light exposure. The data suggest that Ugdh is regulated via an osmoticum-dependent pathway, possibly related to the availability of osmotically active carbohydrate precursors to UDP-glucose, a substrate of UGDH.
DOI: 10.1016/s0167-4781(02)00292-0
PubMed: 12031484
Affiliations:
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Kleczkowski</name></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Binding Sites (MeSH)</term><term>Cell Wall (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (biosynthesis)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (isolation & purification)</term><term>Gene Library (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Pectins (metabolism)</term><term>Plant Leaves (metabolism)</term><term>Polyethylene Glycols (MeSH)</term><term>Polysaccharides (metabolism)</term><term>Sorbitol (MeSH)</term><term>Sucrose (MeSH)</term><term>Trees (MeSH)</term><term>Uridine Diphosphate Glucose Dehydrogenase (biosynthesis)</term><term>Uridine Diphosphate Glucose Dehydrogenase (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (biosynthèse)</term><term>ADN complémentaire (composition chimique)</term><term>ADN complémentaire (isolement et purification)</term><term>Arbres (MeSH)</term><term>Banque de gènes (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Pectine (métabolisme)</term><term>Polyosides (métabolisme)</term><term>Polyéthylène glycols (MeSH)</term><term>Saccharose (MeSH)</term><term>Sites de fixation (MeSH)</term><term>Sorbitol (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>UDP glucose dehydrogenase (biosynthèse)</term><term>UDP glucose dehydrogenase (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA, Complementary</term><term>Uridine Diphosphate Glucose Dehydrogenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Uridine Diphosphate Glucose Dehydrogenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN complémentaire</term><term>UDP glucose dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN complémentaire</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>UDP glucose dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Pectins</term><term>Plant Leaves</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Paroi cellulaire</term><term>Pectine</term><term>Polyosides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Binding Sites</term><term>Cloning, Molecular</term><term>Gene Library</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Polyethylene Glycols</term><term>Sorbitol</term><term>Sucrose</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Banque de gènes</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Gènes de plante</term><term>Polyéthylène glycols</term><term>Saccharose</term><term>Sites de fixation</term><term>Sorbitol</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant UDP-glucose dehydrogenase (UGDH) is an important enzyme in the formation of hemicellulose and pectin, the components of newly formed cell walls. A cDNA clone (Ugdh) corresponding to UGDH was isolated from a cDNA library prepared from cambial zone of poplar (Populus tremula x tremuloides). Within the 1824-nucleotide (nt)-long clone, an open reading frame encoded a protein of 481 amino acids (aa), with a calculated molecular weight of 53.1 kDa. The derived aa sequence showed 90% and 63% identity with UGDHs from soybean and bovine liver, respectively, and had highly conserved aa motifs believed to be of importance for nt binding and catalytic efficiency. In poplar, the Ugdh corresponds to one or two genes, as found by genomic Southern analysis. The gene was expressed predominantly in differentiating xylem and young leaves, with little expression in the phloem zone of the stem. The expression pattern matched that of UGDH protein, as found by immunoblotting. In leaves, the Ugdh expression was upregulated by a short-term feeding with sucrose, sorbitol and polyethylene glycol, and this effect was to some extent mimicked by light exposure. The data suggest that Ugdh is regulated via an osmoticum-dependent pathway, possibly related to the availability of osmotically active carbohydrate precursors to UDP-glucose, a substrate of UGDH.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12031484</PMID><DateCompleted><Year>2002</Year><Month>09</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-3002</ISSN><JournalIssue CitedMedium="Print"><Volume>1576</Volume><Issue>1-2</Issue><PubDate><Year>2002</Year><Month>Jun</Month><Day>07</Day></PubDate></JournalIssue><Title>Biochimica et biophysica acta</Title><ISOAbbreviation>Biochim Biophys Acta</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene of hemicellulose/pectin formation.</ArticleTitle><Pagination><MedlinePgn>53-8</MedlinePgn></Pagination><Abstract><AbstractText>Plant UDP-glucose dehydrogenase (UGDH) is an important enzyme in the formation of hemicellulose and pectin, the components of newly formed cell walls. A cDNA clone (Ugdh) corresponding to UGDH was isolated from a cDNA library prepared from cambial zone of poplar (Populus tremula x tremuloides). Within the 1824-nucleotide (nt)-long clone, an open reading frame encoded a protein of 481 amino acids (aa), with a calculated molecular weight of 53.1 kDa. The derived aa sequence showed 90% and 63% identity with UGDHs from soybean and bovine liver, respectively, and had highly conserved aa motifs believed to be of importance for nt binding and catalytic efficiency. In poplar, the Ugdh corresponds to one or two genes, as found by genomic Southern analysis. The gene was expressed predominantly in differentiating xylem and young leaves, with little expression in the phloem zone of the stem. The expression pattern matched that of UGDH protein, as found by immunoblotting. In leaves, the Ugdh expression was upregulated by a short-term feeding with sucrose, sorbitol and polyethylene glycol, and this effect was to some extent mimicked by light exposure. The data suggest that Ugdh is regulated via an osmoticum-dependent pathway, possibly related to the availability of osmotically active carbohydrate precursors to UDP-glucose, a substrate of UGDH.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Johansson</LastName><ForeName>Henrik</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 901-87, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sterky</LastName><ForeName>Fredrik</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Amini</LastName><ForeName>Bahram</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Lundeberg</LastName><ForeName>Joakim</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Kleczkowski</LastName><ForeName>Leszek A</ForeName><Initials>LA</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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Glycols</NameOfSubstance></Chemical><Chemical><RegistryNumber>506T60A25R</RegistryNumber><NameOfSubstance UI="D013012">Sorbitol</NameOfSubstance></Chemical><Chemical><RegistryNumber>57-50-1</RegistryNumber><NameOfSubstance UI="D013395">Sucrose</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>89NA02M4RX</RegistryNumber><NameOfSubstance UI="D010368">Pectins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.22</RegistryNumber><NameOfSubstance UI="D014533">Uridine Diphosphate Glucose Dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001665" 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IdType="doi">10.1016/s0167-4781(02)00292-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Amini, Bahram" sort="Amini, Bahram" uniqKey="Amini B" first="Bahram" last="Amini">Bahram Amini</name><name sortKey="Kleczkowski, Leszek A" sort="Kleczkowski, Leszek A" uniqKey="Kleczkowski L" first="Leszek A" last="Kleczkowski">Leszek A. Kleczkowski</name><name sortKey="Lundeberg, Joakim" sort="Lundeberg, Joakim" uniqKey="Lundeberg J" first="Joakim" last="Lundeberg">Joakim Lundeberg</name><name sortKey="Sterky, Fredrik" sort="Sterky, Fredrik" uniqKey="Sterky F" first="Fredrik" last="Sterky">Fredrik Sterky</name></noCountry><country name="Suède"><noRegion><name sortKey="Johansson, Henrik" sort="Johansson, Henrik" uniqKey="Johansson H" first="Henrik" last="Johansson">Henrik Johansson</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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